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Table 3 Expression Stability of RG Evaluated by BestKeeper Software

From: Identification of valid reference genes during the differentiation of human myoblasts

 

PPIA

RPLPO

TBP

B2M

GAPDH

ACTB

BK

n = 6

BK

n = 3

n

10.0

10.0

10.0

10.0

10.0

10.0

10.0

10.0

SD [± CP]

0.70

0.83

0.99

1.05

1.16

1.12

0.98

0.83

CV [% CP]

2.9

4.5

4.0

5.8

6.0

7.5

5.0

3.7

  1. Descriptive statistics of six RG based on their crossing point (CP) values. In the two last columns the BestKeeper (BK) index is computed together with the same descriptive parameters, either for six genes (RPLPO, TBP, GAPDH, B2M, ACTB and PPIA) or for three genes after removal of GAPDH, B2M and ACTB. Abbreviations: CV [%CP]: the coefficient of variance expressed as a percentage on the CP level; SD [± CP]: the standard deviation of the CP; SD [± x-fold]: standard deviation of the absolute regulation coefficients; Results from three independent experiments are shown.