Identification of valid reference genes during the differentiation of human myoblasts

BMC Molecular Biology

Table 2 Expression Stability of RG Evaluated by NormFinder Software

Gene name	N and D		Medium N		Medium D
	Stability value*	SE	Stability value*	SE	Stability value*	SE
RPLPO	0.093	0.069	0.071	0.079	0.050	0.135
TBP	0.109	0.065	0.100	0.072	0.050	0.135
PPIA	0.318	0.085	0.201	0.087	0.335	0.131
GAPDH	0.374	0.096	0.376	0.138	0.393	0.148
B2M	0.258	0.075	0.181	0.083	0.125	0.089
ACTB	0.344	0.090	0.205	0.088	0.348	0.135
Best gene:	RPLPO		RPLPO		RPLPO and TBP

Candidate reference genes for normalization are listed according to their expression stability calculated by the NormFinder program (14). The analysis of the expression stability was performed taken the expression of the RG from RNA samples of myoblasts. The analysis was performed using myoblasts cultured in normal medium (N), or differentiation medium (D). Additionally, the analysis of the combination of N and D together is shown. *High expression stability is indicated by a low stability value as an estimate of the combined intra- and intergroup variation of the individual gene. SE stands for standard error. Representative results from three experiments.

ISSN: 1471-2199