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Table 1 Amplification efficiencies.

From: Identification of valid reference genes during the differentiation of human myoblasts

RG

GAPDH

TBP

RPLPO

Mean

1.97

1.94

1.94

SEM

0.0856

0.0364

0.0336

n

4

3

4

RG

B2M

ACTB

PPIA

Mean

1.95

1.94

1.94

SEM

0.0426

0.0290

0.0363

n

4

3

3

GOI

ACTA1

MYOG

MYH3

Mean

1.98

1.99

2.00

SEM

0.0141

0.0677

0.0504

n

3

4

3

  1. Mean of the amplification efficiencies of six reference genes and three genes of interest (GOI). Efficiency calculation was performed from the slopes of the calibration curve according to the equation E = 10 [-1/slope]. Abbreviations: RG, reference genes; GOI, genes of interest; SEM, arithmetic, standard error of the mean; n, number of PCR runs.