Maternally derived eGFP deposited in embryos of Vas2GFP transgenic mosquitoes. (A) Brightfield (upper panels) and fluorescent micrographs (lower panels) of embryos deriving from heterozygous transgenic Vas2GFP mothers (left) or Vas2GFP fathers (right) crossed to wild type. (B) RT-PCR analysis of eGFP transcript deposition in laid embryos deriving from transgenic Vas2GFP mothers or Vas2GFP fathers in a time course from 1 hour post-oviposition until 24 hours post-oviposition. C) Larvae deriving from either heterozygous Vas2GFP mothers crossed to wild type males or heterozygous Vas2GFP fathers crossed to wild type females scored for eGFP (maternal deposition) and RFP fluorescence (segregation of transgene). Scoring was performed in all hatched larvae immediately upon hatching (L1 larval stage) and 5 days later (L2 larval stage).