Figure 2

Transgenic constructs and expression pattern of eGFP in transgenic adult mosquitoes. (A) Schematic diagram of the Anopheles gambiae vasa locus and the Vas1-GFP and Vas2-GFP transgenic constructs. (B) RT-PCR expression analysis of transgenic lines. We used cDNA from transgenic adult Vas1GFP and Vas2GFP mosquitoes of head and thorax (HT), abdomen (AB) ovaries (OV) and testis (TE) to determine expression of endogenous vasa, eGFP and the S7 control gene.