Figure 7
From: Identification of a novel nucleolin related protein (NRP) gene expressed during rat spermatogenesis
NRP supports rDNA transcription. A) Western blot analysis of nucleolin in GC1-Spg cells after siRNA and antisense oligo transfection using rabbit polyclonal nucleolin antibody. Panel 1, RAG cell line (siRNA transfected) and panel 2, GC1-Spg (siRNA transfected) and panel 3 GC1-Spg (antisense oligo transfected) – lane 1, (24 hr); lane 2, (36 hr); lane 3, (48 hr); and lane 4 (60 hr). B) Immunolocalisation of fibrillarin in GC1-Spg cell line after siRNA and antisense oligo transfection. GC1-Spg cells after 60 hour post transfection with siRNA and antisense oligo were fixed and stained with anti-fibrillarin antibody and further with anti-goat secondary antibody conjugated with alexa red. Panel1, (siRNA transfected) and panel 2, (antisense oligo transfected) and panel 3, (control). C) Nuclear run on transcription in GC1-Spg cell line after siRNA transfection. Cells after 60 hours post transfection with siRNA were harvested and nuclear run-on transcription was carried out as described earlier. Lane 1, untransfected and lane 2, transfected. D) Nuclear run on transcription in GC1-Spg cell line after antisense oligo transfection. Cells after 60 hours post transfection with antisense oligo were harvested and nuclear run-on transcription was carried out. Lane 1, untransfected and lane 2, transfected.