Figure 2

Expression of fibrillarin and UBF in spermatogenic cells. A) Immunolocalisation of fibrillarin using goat polyclonal anti-fibrillarin. Nucleus was stained with DAPI. HeLa cells, (panel a-b); gametic diploid cells, (panel c-d); meiotic spermatocytes, (panel e-f); and spermatid cells, (panel g-h). B) Immunolocalisation of UBF using rabbit polyclonal UBF antibody followed by goat anti-rabbit conjugated with Alexa 568 as secondary antibodies. Nucleus was stained with DAPI. HeLa cells, (panel a-b); gametic diploid cells, (panel c-d); meiotic spermatocytes, (panel e-f); spermatid cells, (panel g-h). C) Realtime PCR of nucleolin, Fibrillarin and UBF. Differences (n-fold) in expression of nucleolin, fibrillarin and UBF were compared in meiotic spermatocytes, spermatids and gametic diploid cells keeping the low expressing cells as the reference. Each value plotted represents an average of 3 independent triplicate experiments ± standard deviation. 2n, diploid cell (striped bar); 4n, meiotic spermatocytes (black bar with white dots); and n, haploids cells (white bar with black dots).