Table 3 Reference genes, qPCR primers and reaction parameters.
Gene | Forward primer (5'-3') | Reverse primer (5'-3') | Amplicon size | Q-PCR Linear correlation coefficient | Q-PCR Efficiency (%) | Average Ct ± SD |
|---|---|---|---|---|---|---|
Candidate reference genes | ||||||
   coaE | ACT ATC GCC CAT TGC TGG AT | TTG GTA ATG ATC CAG TCG GC | 169 | 0.999 | 93 | 25.50 ± 0.69 |
   era | CAT GGA TGA GAT CAA GAG CG | GTA TCC CGA GAA TCT GAT CC | 174 | 0.999 | 94 | 22.56 ± 0.21 |
   gmk | ATG GCA CTA GTG AAC CTT GG | AAT GAC CGT CTC CGA ATC CT | 197 | 0.998 | 92 | 24.49 ± 0.50 |
   gyrA | TAC CTC GAT TAC GCC ATG AG | TGT CAT AGA CAG CGG TAT CG | 202 | 0.998 | 95 | 26.24 ± 0.45 |
   map | TTA CCA CCG ATG AAC TGG AC | AGC CAT CCT TGA TAA CCG TG | 222 | 0.999 | 94 | 20.64 ± 0.29 |
   nth | ATC GTC TGG GAC TGT TCA AC | TAT TGA GGA CGA CAT TGG CG | 153 | 0.997 | 94 | 25.31 ± 0.40 |
   rplI | GAT GCC ATG GTT ACG ATT GC | GAC CAT CAC ATC CAG TTC GA | 210 | 1.000 | 92 | 21.68 ± 0.51 |
   rpoC | AAT GCG GTG TTG AGG TAA CC | AGG TAC TGG TCT TCG GTA AG | 238 | 0.997 | 95 | 20.79 ± 0.23 |
   trpS | TCT GCT CAT CGA ATG GTT GG | GAA TGT CTG CCG TCA TCA AC | 230 | 0.999 | 89 | 23.86 ± 0.70 |
Reference genes used in prior studies | ||||||
   alaS | CTG GAA TCT GGT CTT CAT GC | GCT TGA AGA GAT CGG TGT CA | 152 | 1.000 | 94 | 22.35 ± 0.39 |
   recA | CCG CCA ACA TTT CCC GGA CC | ACG CCG CGG TCC ACC AGT TC | 301 | 0.998 | 98 | 29.41 ± 0.88 |
   rrs | ACA CTG GGA CTG AGA CAC GG | ACC GCC TAC GCA CCC TTT AC | 277 | 0.999 | 95 | 8.93 ± 0.27 |
Differentially expressed target genes | ||||||
   cbbOIa | ACG GCA TTG AGC TAT ACC G | TCC GGG TCT AGT AGT GCA T | 265 | 0.996 | 97 | 21.55 ± 0.72 |
   cyoB | CAA TTA CAT GGT CCA CAA CA | AGC GTA TAC ACC ACG ATA CC | 300 | 0.995 | 92 | 28.11 ± 2.17 |
   mntH | ATA TCG GTG CCG TCA TCA TG | GAG AGC TGA TGC ACC GTA TT | 217 | 0.996 | 97 | 21.92 ± 2.02 |
   sdrAI | GTT TGG GTG CTG AAG TAG TG | GCA ACA GTG GCA AAC AGG C | 255 | 0.995 | 93 | 23.61 ± 2.82 |