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Figure 4 | BMC Molecular Biology

Figure 4

From: Selection and evaluation of reference genes for improved interrogation of microbial transcriptomes: case study with the extremophile Acidithiobacillus ferrooxidans

Figure 4

Comparison of different normalization strategies. Mean relative expression levels of four differentially expressed genes using different normalization strategies. Black: stable reference genes used individually, light grey: geometric mean of three stable reference genes selected by geNorm (NF1: rpoC, map and alaS), dark grey: geometric mean of four stable reference genes selected by geNorm (NF2: rpoC, map and era) and white: classical reference genes used individually. (*) Significantly different according to a two tailed unpaired t-test at 95% confidence.

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