Table 2 List of primers and reference genes under investigation
Gene | Gene's symbol | Accession number | Forward primer | Reverse primer | Amplicon length | Primer efficiency |
|---|---|---|---|---|---|---|
Ubiquitin c | ubc | FM882773 | GCGTCATAAGTTGCGGAGA | TCTTGGCCTTCACATTTTCA | 106 | 100% |
Fructose bisphosphate aldolase | ald | FM882346 | TATGTCCCAGCGTTGTGCT | TGGCACCAGACCATTCATT | 166 | Non-specific products |
NADH dehydrogenase subunit 1 | mt-nd1 | FM882393 | CGGGCTGTTGCTCAAACTA | TTCGACAAAATCTTCCAATCT | 102 | 100% |
Tubulin alpha | tuba | FM882619 | CCTGCTGGGAGCTGTATTGT | ACAACAGTGGGCTCCAAATC | 169 | 94% |
NADH dehydrogenase subunit 4L | mt-nd4L | FM882472 | TTCTTGGTAGCTTCTGTGTGTG | TAGTCGGAACCATGTGATCG | 80 | 100% |
Tubulin beta | tubb | FM882322 | ACCTCAGCCTGGTCATCATC | GGCTTTCCTCCACTGGTACA | 165 | 84% |
Cysteine protease 1 | cp1 | FM882273 | GTTGAGCAGCACATGAAGGA | CGAACTCCTCAGAGGTCAGG | 91 | 94% |
Cytochrome b | mt-cyb | FM882286 | GGACACTGCATGCTAATGGA | AGGCAGCAGCCATAGTCAAG | 144 | 91% |
Acyl carrier | mt-acp | FM882501 | GATGTGGCGATTTGCTATCC | TTCTCCGGGTTGATCTTGTC | 175 | 93% |
Myosin 1-light chain | mlc1 | FM882394 | AAGGATGAGGTTGACGCCTA | ACCCTGGTCCTTGTCCTTCT | 174 | Presence of primer dimers |
60s ribosomal protein L15 | rplL5 | FM882400 | AAGCAGGGATACGCCATCTA | AGCTTCAGCTCGTTCACTCC | 116 | 84% |
Glyceraldehyde-3-phosphate dehydrogenase | gapdh | FM882736 | TCTGCGGCTTACTTGTCCTT | ACTCGCACTCGAGCATCTTT | 154 | Non-specific products |
Elongation Factor 1 alpha | ef1a | FM882302 | GCCACAGGGATTTCATCAAG | TGGAGATACCAGCCTCGAAC | 105 | 100% |
Actin alpha | act | FM882301 | CAGTCCAAGCGTGGTATCCT | CGCACGCAGCTCGTTGTAGA | 114 | 100% |