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Figure 1 | BMC Molecular Biology

Figure 1

From: A strategy for enrichment of claudins based on their affinity to Clostridium perfringens enterotoxin

Figure 1

Strategy for affinity purification using GST- CPE 116–319 as "bait". For affinity purification, one cell population is grown in medium containing [12C]arginine and [12C]lysine, whereas the other is grown in medium supplemented with [13C]arginine and [13C]lysine. Bound proteins of both pull-down fractions are combined and separated by SDS-PAGE. After tryptic in-gel digestion, proteins are identified by LC-MS/MS, and the ratios of their abundance are quantified from MS peak intensities.

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