Elongation factor eEF1B modulates functions of the release factors eRF1 and eRF3 and the efficiency of translation termination in yeast

Table 2 Plasmids

Plasmid	Characteristics	Source
pCM183-SUP45	Centromeric TRP1 vector containing SUP45 under the control of regulatable tetO₂ promoter	[20]
YEplac195	Multicopy URA3 vector	[26]
YEplac195-SUP45	Same as YEplac195, but with SUP45	This work
YEplac195-TEF2	Same as YEplac195, but with TEF2	This work
YEplac195-TEF4	Same as YEplac195, but with wild type TEF4	This work
YEplac195-TEF5	Same as YEplac195, but with wild type TEF5	This work
YEplac195-TEF5Δi	Same as YEplac195, but with TEF5 without intron (TEF5-Δi)	This work
YEplac181	Multicopy LEU2 vector	[26]
YEplac181-TEF2	Same as YEplac181, but with TEF2	This work
YEplac181-TEF5Δi	Same as YEplac181, but with TEF5 without intron (TEF5-Δi)	This work
YEplac181-TEF3	Same as YEplac181, but with TEF3	This work
YEplac181-TEF4Δi	Same as YEplac181, but with TEF4 without intron (TEF4-Δi)	This work
YEplac181-SUP35C	Same as YEplac181, but with SUP35-C	This work
pRS315	Centromeric LEU2 vector	[27]
pRS315-SUP35	Same as pRS315, but with SUP35	[20]
pRS315-sup35-168	Same as pRS315, but with the sup35-168^tsallele	This work
pRS315-sup35-196	Same as pRS315, but with the sup35-196^tsallele	This work
pRS315-SUP45	Same as pRS315, but with SUP45	[17]
pRS315-sup45-sl23	Same as pRS315, but with the sup45-sl23^tsallele	[17]
pRG416-SUP35C	Centromeric URA3 vector containing SUP35-C	[7]
pRG415-SUP45	Centromeric URA3 vector containing SUP45	[19]
pBSS35::H3	Bacterial vector containing the sup35::HIS3 disruption allele	This work

ISSN: 1471-2199