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Table 2 Plasmids

From: Elongation factor eEF1B modulates functions of the release factors eRF1 and eRF3 and the efficiency of translation termination in yeast

Plasmid Characteristics Source
pCM183-SUP45 Centromeric TRP1 vector containing SUP45 under the control of regulatable tetO2 promoter [20]
YEplac195 Multicopy URA3 vector [26]
YEplac195-SUP45 Same as YEplac195, but with SUP45 This work
YEplac195-TEF2 Same as YEplac195, but with TEF2 This work
YEplac195-TEF4 Same as YEplac195, but with wild type TEF4 This work
YEplac195-TEF5 Same as YEplac195, but with wild type TEF5 This work
YEplac195-TEF5Δi Same as YEplac195, but with TEF5 without intron (TEF5-Δi) This work
YEplac181 Multicopy LEU2 vector [26]
YEplac181-TEF2 Same as YEplac181, but with TEF2 This work
YEplac181-TEF5Δi Same as YEplac181, but with TEF5 without intron (TEF5-Δi) This work
YEplac181-TEF3 Same as YEplac181, but with TEF3 This work
YEplac181-TEF4Δi Same as YEplac181, but with TEF4 without intron (TEF4-Δi) This work
YEplac181-SUP35C Same as YEplac181, but with SUP35-C This work
pRS315 Centromeric LEU2 vector [27]
pRS315-SUP35 Same as pRS315, but with SUP35 [20]
pRS315-sup35-168 Same as pRS315, but with the sup35-168tsallele This work
pRS315-sup35-196 Same as pRS315, but with the sup35-196tsallele This work
pRS315-SUP45 Same as pRS315, but with SUP45 [17]
pRS315-sup45-sl23 Same as pRS315, but with the sup45-sl23tsallele [17]
pRG416-SUP35C Centromeric URA3 vector containing SUP35-C [7]
pRG415-SUP45 Centromeric URA3 vector containing SUP45 [19]
pBSS35::H3 Bacterial vector containing the sup35::HIS3 disruption allele This work