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Table 1 S. cerevisiae strains

From: Elongation factor eEF1B modulates functions of the release factors eRF1 and eRF3 and the efficiency of translation termination in yeast

Strain Genotype Source
33G-D373 MATα ura3-52 leu2-3,112 trp1-289 his7-1 lys9-A21 ade2-144,717 pheA-11 [25]
33G-D373-rSL23 MATα ura3-52 leu2-3,112 trp1-289 his7-1 lys9-A21 ade2-144,717 pheA-11 sup45-sl23 ts [20]
33G-D373-r36 MATα ura3-52 leu2-3,112 trp1-289 his7-1 lys9-A21 ade2-144,717 pheA-11 sup45-36 ts [20]
33G-D373-rSL23-r35C MATα ura3-52 leu2-3,112 trp1-289 his7-1 lys9-A21 ade2-144,717 pheA-11 sup45-sl23 ts SUP35-C [20]
33G-D373-r36-r35C MATα ura3-52 leu2-3,112 trp1-289 his7-1 lys9-A21 ade2-144,717 pheA-11 sup45-36 ts SUP35-C [20]
8V-H80 MATα ade1-14 his7-1 leu2-3,112 ura3-52 trp1-289 lys2-A12 This work
8V-H80-168 MATα ade1-14 his7-1 leu2-3,112 ura3-52 trp1-289 lys2-A12 sup35-168 This work
8V-H80-196 MATα ade1-14 his7-1 leu2-3,112 ura3-52 trp1-289 lys2-A12 sup35-196 This work
BY4741 MAT a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Open Biosystems
BY4741-ΔSUP35 MAT a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 sup35::HIS3 [pRG416-SUP35C] This work
50V-H78 MATα ade1-14 ura3-52 leu2-3,112 his3-D200 his5-2 lys1-1 met8-1 ilv1-1 SUP4 This work
  1. Plasmid that supports viability of the BY4741-ΔSUP35 strain is indicated in square brackets.