Figure 4From: Elongation factor eEF1B modulates functions of the release factors eRF1 and eRF3 and the efficiency of translation termination in yeastHigh dosage of TEF5 reduces efficiency of UAA readthrough in the sup35 mutants. The strains 8V-H80-168 (sup35-168ts) and 8V-H80-196 (sup35-196ts) were transformed with one of the multicopy plasmids YEplac181-TEF5Δi (TEF5-Δi↑), YEplac181-SUP35C (SUP35C↑) or YEplac181 (empty vector) and then with the appropriate dual luciferase reporter plasmids. The transformants with plasmid pairs were grown and readthrough measured as described in legend to Figure 3 and in Methods. The difference in UAA readthrough in the mutant sup35-196tsstrain with the YEplac181-TEF5Δi and YEplac181 plasmids is statistically significant (P ≤ 0.1).Back to article page