Skip to main content
Figure 5 | BMC Molecular Biology

Figure 5

From: Optimal use of tandem biotin and V5 tags in ChIP assays

Figure 5

A) Chromatin from I/11 cells prepared with SDS in sonication buffer. Binding of RAD 21 and negative control (NC) to a β-globin site and the amylase gene (control for non-specific binding). B) Chromatin from I/11 cells prepared without SDS in sonication buffer. Binding of RAD 21 to specific site in β-globin locus is significantly improved in comparison to the result in panel A. C) Chromatin from I/11 cells prepared with SDS in sonication buffer. Binding of RAD 21 and negative control (NC) to a β-globin site and the necdin gene (control for non-specific binding). D) Chromatin from I/11 cells prepared without SDS in sonication buffer. Binding of RAD 21 to specific site in β-globin locus is significantly improved in comparison to result in panel C. Note that the binding of negative control (NC) to β-globin locus site is reduced.

Back to article page