Table 1 List of SHBG and s18 (control gene) primers used for RT-PCR
Target | Sequence | Annealing temp | Cycles |
|---|---|---|---|
Exon 3 upper Exon 8 lower | 5' CACGGCTGGATGATGGGA 3' 5' AGGGGGGTTCTTAGGTGGAGC 3' | 59°C | 39 |
Exon 1 upper Exon 5 lower | 5' ATGGAGAGCAGAGGCCCACTG 3' 5' TGAGATCTCGGCCTGTTTGTC 3' | 59°C | 39 |
Exon 1A upper Exon 6 lower | 5' TTCAAAGGCTCCCCCGCAGTGC 3' 5' GGAGACTGAGCCAAGATGGGT 3' | 60°C | 39 |
Exon 1B upper Exon 3 lower | 5' TGAAGAGCCTGAGAGAGCG 3' 5' AGGCCTGCCGTCTCGAAGTCCC 3' | 61°C | 39 |
Exon 1B upper Exon 8 lower | 5' TGAAGAGCCTGAGAGAGCG 3' 5' AGGGGGGTTCTTAGGTGGAGC 3' | 59°C | 39 |
Exon 1C upper Exon 3 lower | 5' ACCTTCTTCACTGATCTTCAC 3' 5' AGGCCTGCCGTCTCGAAGTCCC 3' | 57°C | 39 |
Exon 1C upper Exon 5 lower | 5' ACCTTCTTCACTGATCTTCAC 3' 5' TGAGATCTCGGCCTGTTTGTC 3' | 58°C | 35 |
Exon 1C upper Exon 8 lower | 5' ACCTTCTTCACTGATCTTCAC 3' 5' TGGCTTCTGTTCAGGGCC 3' | 58°C | 39 |
Exon 1D upper Exon 3 lower | 5' TTCCCAAAGGGACCGTGTG 3' 5' AGGCCTGCCGTCTCGAAGTCCC 3' | 58°C | 39 |
Exon 1E upper Exon 3 lower | 5' CGTCGTAAATGGATTGACC 3' 5' AGGCCTGCCGTCTCGAAGTCCC 3' | 57°C | 39 |
S18 upper S18 lower | 5' GATGGGCGGGGGAAAAT 3' 5' CTTGTACTGGCGTGGATTCTGC 3' | 55–60°C | 29 |