Figure 8

Amplification of the full-length exon 1C SHBG transcript. A) Scheme of the nested PCR designed to amplify the full-length exon 1C transcript from cDNA of imr 32 and CW 9019 cell lines, using primers directed against exons 1C and 8 (first round) and against exons 1C and 5 (second round). B) A major 1.2 kb band (asterisk) was obtained in the first round. C) A 635 bp PCR product resulted from the second round (asterisk). The direct sequencing of this product confirmed the SHBG exon 1C sequence. imr 32: cDNA of imr 32 cells used as positive control. Ctrl-: negative control using water instead of cDNA.