Figure 1
Analysis of the annotated 5' end of the human SHBG gene. A) The FirstEF program predicts three different sequences as potential first exons (exon predictions 1 to 3). The potential novel SHBG first exon is shown in bold print (exon prediction 3) and promoter 1 (P1) and alternative promoter (PA) are indicated. B) Exon 1B sequence (overlayed in gray) and primers (i and ii) (overlined) used for PCR amplification. C) RT-PCR amplification of the 1.2 kb exon 1B transcript in LNCaP cells and human prostatic tissue. D) RT-PCR amplification of the exon 1B transcript using primer (i). Arrow: expected 300 bp band. Upper band: sequence containing exons 1B-1-2-3. Ctrl-: negative control using water instead of cDNA. E) Comparison of the splicing patterns of exons 1A and 1B between humans and Macaca.