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Table 1 The numbers of X-gal positive striatal cells from rats sacrificed at various times after microinjection of pNFHlac packaged using specific combinations of mutated HSV-1 proteins

From: Improved long-term expression from helper virus-free HSV-1 vectors packaged using combinations of mutated HSV-1 proteins that include the UL13 protein kinase and specific components of the VP16 transcriptional complex

 

Purified titersa

Average X-gal positive cells per rat

Time after gene transfer

Relative efficiency of gene

transfer b

Packaging condition

VG/ml

IVP/ml

VG/IVP

4 days

2 wks

1 month

2 months

3 months

 

cΔul46&47, ul13g

6.4 × 107

5.0 × 106

10.1

1023 ± 23

680 ± 49

480 ± 11

319 ± 5

155 ± 30

5.7

VP16in14, gene12, ul13g

7.6 × 106

7.2 × 105

10.1

201 ± 8

119 ± 9

65 ± 6

44 ± 4

23 ± 6

2.3

VP16in14, gene12

1.2 × 107

1.8 × 106

6.7

343 ± 17

104 ± 4

65 ± 15

36 ± 8

16 ± 2

1.6

ul13g

2.0 × 107

1.8 × 106

11.1

393 ± 17

197 ± 6

99 ± 16

47 ± 19

19 ± 5

1.8

Δul46&47d

3.1 × 107

3.0 × 106

10

2,532 ± 93

980 ± 88

616 ± 58

315 ± 17

92 ± 11

9.9

wt d

6.3 × 107

5.2 × 106

12

445 ± 39

63 ± 9

12 ± 2

0 ± 0

0 ± 0

1.0

  1. Three rats were analyzed for condition and time point; the means ± SDs are shown.
  2. aThe titers of each vector stock after purification and concentration. VG/ml is vector genomes/ml; IVP/ml is infectious vector particles/ml.
  3. bThe efficiency of gene transfer is the number of positive cells at 4 days/the amount of vector injected. The relative efficiency of gene transfer is the efficiency of gene transfer with a specific condition/the efficiency of gene transfer with wt packaging.
  4. cVector stock diluted 3.3-fold before gene transfer.
  5. dFor these two packaging conditions, the titers and the X-gal cell counts for 4 days through 2 months were previously reported in [19] and are included here only to support comparisons. The X-gal cell counts at 3 months have not been previously reported.