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Table 1 The numbers of X-gal positive striatal cells from rats sacrificed at various times after microinjection of pNFHlac packaged using specific combinations of mutated HSV-1 proteins

From: Improved long-term expression from helper virus-free HSV-1 vectors packaged using combinations of mutated HSV-1 proteins that include the UL13 protein kinase and specific components of the VP16 transcriptional complex

  Purified titersa Average X-gal positive cells per rat Time after gene transfer Relative efficiency of gene transfer b
Packaging condition VG/ml IVP/ml VG/IVP 4 days 2 wks 1 month 2 months 3 months  
cΔul46&47, ul13g 6.4 × 107 5.0 × 106 10.1 1023 ± 23 680 ± 49 480 ± 11 319 ± 5 155 ± 30 5.7
VP16in14, gene12, ul13g 7.6 × 106 7.2 × 105 10.1 201 ± 8 119 ± 9 65 ± 6 44 ± 4 23 ± 6 2.3
VP16in14, gene12 1.2 × 107 1.8 × 106 6.7 343 ± 17 104 ± 4 65 ± 15 36 ± 8 16 ± 2 1.6
ul13g 2.0 × 107 1.8 × 106 11.1 393 ± 17 197 ± 6 99 ± 16 47 ± 19 19 ± 5 1.8
Δul46&47d 3.1 × 107 3.0 × 106 10 2,532 ± 93 980 ± 88 616 ± 58 315 ± 17 92 ± 11 9.9
wt d 6.3 × 107 5.2 × 106 12 445 ± 39 63 ± 9 12 ± 2 0 ± 0 0 ± 0 1.0
  1. Three rats were analyzed for condition and time point; the means ± SDs are shown.
  2. aThe titers of each vector stock after purification and concentration. VG/ml is vector genomes/ml; IVP/ml is infectious vector particles/ml.
  3. bThe efficiency of gene transfer is the number of positive cells at 4 days/the amount of vector injected. The relative efficiency of gene transfer is the efficiency of gene transfer with a specific condition/the efficiency of gene transfer with wt packaging.
  4. cVector stock diluted 3.3-fold before gene transfer.
  5. dFor these two packaging conditions, the titers and the X-gal cell counts for 4 days through 2 months were previously reported in [19] and are included here only to support comparisons. The X-gal cell counts at 3 months have not been previously reported.