| Purified titersa | Average X-gal positive cells per rat Time after gene transfer | Relative efficiency of gene transfer b | ||||||
---|---|---|---|---|---|---|---|---|---|
Packaging condition | VG/ml | IVP/ml | VG/IVP | 4 days | 2 wks | 1 month | 2 months | 3 months | Â |
cΔul46&47, ul13g | 6.4 × 107 | 5.0 × 106 | 10.1 | 1023 ± 23 | 680 ± 49 | 480 ± 11 | 319 ± 5 | 155 ± 30 | 5.7 |
VP16in14, gene12, ul13g | 7.6 × 106 | 7.2 × 105 | 10.1 | 201 ± 8 | 119 ± 9 | 65 ± 6 | 44 ± 4 | 23 ± 6 | 2.3 |
VP16in14, gene12 | 1.2 × 107 | 1.8 × 106 | 6.7 | 343 ± 17 | 104 ± 4 | 65 ± 15 | 36 ± 8 | 16 ± 2 | 1.6 |
ul13g | 2.0 × 107 | 1.8 × 106 | 11.1 | 393 ± 17 | 197 ± 6 | 99 ± 16 | 47 ± 19 | 19 ± 5 | 1.8 |
Δul46&47d | 3.1 × 107 | 3.0 × 106 | 10 | 2,532 ± 93 | 980 ± 88 | 616 ± 58 | 315 ± 17 | 92 ± 11 | 9.9 |
wt d | 6.3 × 107 | 5.2 × 106 | 12 | 445 ± 39 | 63 ± 9 | 12 ± 2 | 0 ± 0 | 0 ± 0 | 1.0 |