Validation of housekeeping genes for quantitative real-time PCR in in-vivo and in-vitro models of cerebral ischaemia

Table 3 Candidate reference genes

Gene Symbol	Gene Name	TaqMan Assay Number*	Genebank accession
Hprt	Hypoxanthine phosphoribosyltransferase	Rn01527840_m1	NM_012583
Gapdh	Glyceraldehyde-3-phosphate dehydrogenase	Rn99999916_s1	NM_017008
Oaz1	Ornithine decarboxylase antizyme 1	Rn00821793_g1	NM_139081
β2m	Beta-2 microglobulin	Rn00560865_m1	NM_012512
Ppia	Peptidyl-prolyl cis-trans isomerasa	Rn00690933_m1	NM_017101
Rpl13a	Ribosomal Protein L13A	Rn00821946_g1	NM_173340
18S	Eukaryotic 18S rRNA	Hs99999901_s1	X03205.1
Ywhaz	Tyrosine 3-monooxygenase	Rn00755072_m1	NM_013011.3
Sdha	Succinate dehydrogenase complex, subunit A	Rn00590475_m1	NM_130428.1
Gusb	Glucuronidase, beta	Rn00566655_m1	NM_017015.2

*"_m" indicates an assay whose probe spans an exon junction and will not detect genomic DNA. "_s" indicates an assay whose probes and primers are designed within a single exon and, hence, will detect genomic DNA. "_g" indicates an assay that may detect genomic DNA as the assay probe and primers may also be within a single exon.

ISSN: 1471-2199