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Figure 3 | BMC Molecular Biology

Figure 3

From: Validation of housekeeping genes for quantitative real-time PCR in in-vivo and in-vitro models of cerebral ischaemia

Figure 3

Gene expression stability and determination of the optimum number of genes for normalization of the candidate reference genes for the in-vivo ischaemia model using geNorm analysis. (A) Expression stability plot showing average expression stability values M. (B) Pairwise variation analysis to determine the optimal number of reference genes for use in RT qPCR data normalization. The use of the two most stable genes is sufficient for accurate normalization (cutoff 0.15 according to [12])

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