Table 3 Most stable reference genes per treatment calculated by geNorm and Normfinder
Folsomia candida | Orchesella cincta | ||||||||
|---|---|---|---|---|---|---|---|---|---|
Treatment | Top 3 most stable genes | Optimum no. genes | Treatment | Top 3 most stable genes | Optimum no. genes | ||||
Normfinder | GeNorm | Norm finder | GeNorm | Norm finder | GeNorm | Normfinder | GeNorm | ||
Temperature | SDHA | SDHA | 2 | >3 | Temperature | 28s | YWHAZ | >3 | 2 |
ETIF | ETIF | ACTb | ACTb | ||||||
EF1a | EF1a | YWHAZ | 28s | ||||||
Desiccation | SDHA | SDHA | 2 | 3 | Desiccation | ACTb | ACTb | 2 | 2 |
ETIF | ETIF | GAPDH | GAPDH | ||||||
EF1a | GAPDH | EF1a | EF1a | ||||||
Cadmium | YWHAZ | YWHAZ | 3 | 2 | Cadmium | TBa | TBa | >3 | 2 |
SDHA | SDHA | SDHA | YWHAZ | ||||||
GAPDH | GAPDH | YWHAZ | SDHA | ||||||
Phenanthrene | SDHA | SDHA | 2 | 2 | Phenanthrene | n/a | n/a | n/a | n/a |
YWHAZ | YWHAZ | n/a | n/a | ||||||
GAPDH | GAPDH | n/a | n/a | ||||||
pH | UBC | ACTb | >3 | >3 | pH | n/a | n/a | n/a | n/a |
ETIF | CYP | n/a | n/a | ||||||
ACTb | EF1a | n/a | n/a | ||||||
Starvation | n/a | n/a | n/a | n/a | Starvation | ACTb | TBA | >3 | 2 |
n/a | n/a | YWHAZ | YWHAZ | ||||||
n/a | n/a | TBa | SDHA | ||||||
All treatments | n/a | n/a | n/a | n/a | All treatments | ACTb | n/a | n/a | n/a |
n/a | n/a | TBa | n/a | ||||||
n/a | n/a | YWHAZ | n/a | ||||||