BMC Molecular Biology

Table 2 Omitted and remaining genes and their absolute total biases after preselection for Normfinder analysis

From: Reference genes for QRT-PCR tested under various stress conditions in Folsomia candida and Orchesella cincta (Insecta, Collembola)

Folsomia candida						Orchesella cincta
Treatment	Bias treshold	Omitted genes	Bias	Remaining genes	Bias	Treatment	Bias treshold	Omitted genes	Bias	Remaining genes	Bias
Temperature	2.24	YWHAZ	3.78	ETIF	1.01	Temperature	1.08	MT	1.38	TBa	0.24
		HSP70	4.95	UBC	1.05			HSP70	3.49	SDHA	0.32
				SDHA	1.19					YWHAZ	0.45
				EFIa	1.38					EF1a	0.51
				CYP	1.42					28S	0.58
				GAPDH	1.74					ACTb	0.71
				ACTb	2.02					GAPDH	0.87
Desiccation	1.72	GAPDH	2.19	UBC	0.43	Desiccation	0.16	SDHA	0.26	HSP70	0.02
		CP	4.99	CYP	0.70			28S	0.27	GAPDH	0.06
				EFIa	0.70			MT	0.34	YWHAZ	0.07
				ETIF	0.97					EF1a	0.09
				YWHAZ	1.13					TBa	0.10
				SDHA	1.18					ACTb	0.15
				ACTb	1.54
Cadmium	1.44	ETIF	1.64	ACTb	0.20	Cadmium	0.76	GAPDH	0.81	HSP70	0.01
		BCS1	4.31	EFIa	0.32			MT	2.88	ACTb	0.03
				CYP	0.84					SDHA	0.27
				YWHAZ	0.90					28S	0.29
				SDHA	0.95					YWHAZ	0.44
				GAPDH	1.11					TBa	0.50
				UBC	1.24					EF1a	0.60
Phenanthrene	0.85	ETIF	0.89	UBC	0.14	Starvation	0.33	MT	0.35	ACTb	0.07
		BCS1	3.00	ACTb	0.21			EF1a	0.55	HSP70	0.09
				EFIa	0.33			GAPDH	0.58	YWHAZ	0.13
				YWHAZ	0.37			SDHA	0.67	TBa	0.14
				SDHA	0.38					28S	0.16
				GAPDH	0.55	All treatments	1.21	total RNA	2.06	ACTb	0.34
				CYP	0.79			MT	3.33	SDHA	0.53
pH	0.67	EFIa	0.67	ETIF	0.36					TBa	0.55
		GAPDH	0.81	UBC	0.38					28S	0.91
		YWHAZ	0.90	SDHA	0.49					YWHAZ	1.00
		CYP	1.02	ACTb	0.57					EF1a	1.04
				ATPase	0.59					GAPDH	1.05
										HSP70	1.20

Pre-selection of genes prior to Normfinder analysis based on a threshold for maximum allowable bias of 0.13 times the standard deviation of the total absolute bias of a treatment. For each treatment, bias thresholds are given as well as the corresponding omitted and remaining genes and their absolute total biases per treatment (total RNA input was only included in the All treatment analysis for Orchesella cincta).

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