Figure 4

Differential alternative splicing patterns detected in the adult mouse cerebellum. RT-PCR reactions from dissected wildtype and stargazer brain region RNAs (lanes: 1, thalamus; 2, cortex; 3, cerebellum; 4, hippocampus; +, wildtype control; -, heterozygous mutant) reveal differential splicing patterns at exons 14 and 26 in the cerebellum. Amplification of exon 14 shows preference in expression for the alternatively spliced product, ΔE14, at 623 bp, over the typically spliced product at 692 bp. The amplification of exon 26 identifies a shift in splicing resulting in sole expression of the alternatively spliced product, ΔE26, at 238 bp, while the normally spliced product at 292 bp is absent. The DNA marker on the far right separates at 100 bp intervals. Dissected lethargic and tottering samples show similar splicing patterns (data not shown).