Primer sequence | Selection | Mutant |
---|---|---|
5'-ACCGGCCTGGGCCGGCCCG(CA)G ATGGCGGTCTACCAC-3' | BstUI (mutant D58A) | D 58 A D 58 E |
5'-GGTGGTAGACCGCCATC(TG)CG GGCCGGCCCAGGCCGG-3' | Â | Â |
5'-TCCTGGTGGGCTTCGTG(ACGT)(ACGT)(ACGT) CTCAAGGCTCCGGGCAAG-3' | SacI restriction site disappears | E 72 X |
5'-CTTGCCCGGAGCCTTGAG(ACGT)(ACGT)(ACGT) CACGAAGCCCACCAGGA-3' | Â | Â |
5'-CTGGTGGGCTTCGTGGAGCTC(GC)(GC) GGCCCCG GGCAGG-3' | SmaI/ApaI (K74G, K74R) | K 74 G K 74 A K 74 R K 74 P |
5'-CTTGCCCGGGGCC(GC)(GC) GAGCTCCACGAAGCCCACAGG-3' | Â | Â |
5'-GTTACCGTGATAGACCCGGCCA TGGGCACGGGGACCTT-3' | NcoI (V356M) | V 356 M |
5'-AAGGTCCCCGTGCCCAT GGCCGGGTCTATCACGGTAAC-3' | Â | Â |
5'-TGGTGATCCTTGGCGCC CCCCCCTACGACCGGGTG-3' | NarI (N473A) | N 473 A |
5'-CACCCGGTCGTAGGGGGGGGC GCCAAGGATCACCA-3' | Â | Â |