From: Cloning and analysis of a bifunctional methyltransferase/restriction endonuclease TspGWI, the prototype of a Thermus sp. enzyme family
Mutation
REase activity
MTase activity
WT
+++
D58A
__
D58R
E72A
E72D
E72L
E72N
E72R
E72S
K74A
K74G
K74P
K74R
V356M
+
N473A
V356M/N473A