Skip to main content

Table 2 Methylation and restriction activities of TspGWI mutants.

From: Cloning and analysis of a bifunctional methyltransferase/restriction endonuclease TspGWI, the prototype of a Thermus sp. enzyme family

Mutation

REase activity

MTase activity

WT

+++

+++

D58A

__

+++

D58R

__

+++

E72A

__

+++

E72D

+++

+++

E72L

__

+++

E72N

__

+++

E72R

__

+++

E72S

__

+++

K74A

__

+++

K74G

__

+++

K74P

__

+++

K74R

__

+++

V356M

+

+++

N473A

+

__

V356M/N473A

__

__