Table 1 Effect of divalent cations on the enzymatic activities of wt TspGWI and selected mutants of it.
TspGWI variant | No divalent cation | Mg2+ | Ca2+ | Activity |
|---|---|---|---|---|
WT | __ | +++ | __ | REase |
WT | + | + | +++ | MTase |
D58A | __ | __ | __ | REase |
D58A | __ | +++ | +++ | MTase |
N473A | __ | + | __ | REase |
N473A | __ | __ | __ | MTase |