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Figure 8 | BMC Molecular Biology

Figure 8

From: Cloning and analysis of a bifunctional methyltransferase/restriction endonuclease TspGWI, the prototype of a Thermus sp. enzyme family

Figure 8

Effect of AdoMet and AdoHcy on the TspGWI restriction of DNA. Lane M1, 1 kb ladder (selected bands marked); lane M2, 100 bp ladder (selected bands marked); lane 1 (-TspGWI; +Mg2+; -AdoMet; – AdoHcy), control 1 μg of λ DNA; lane 2 (+TspGWI; +Mg2+;-AdoMet; – AdoHcy) λ DNA incubated with 1 u of TspGWI REase in the reaction mixture with Mg2+ and without AdoMet; lane 3 (+TspGWI; +Mg2+; +AdoMet; -AdoHcy), λ DNA incubated with 1 u of TspGWI REase in the reaction mixture with Mg2+ and with AdoMet; lane 4 (+TspGWI; +Mg2+;-AdoMet; + AdoHcy), λ DNA incubated with 1 u of TspGWI REase in the reaction mixture with Mg2+ and with AdoHcy.

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