Figure 3From: Cloning and analysis of a bifunctional methyltransferase/restriction endonuclease TspGWI, the prototype of a Thermus sp. enzyme familySequence alignment between TspGWI and its close homologues in REBASE (BLAST E-value < 1E-40). The PD-(D/E)XK domain with the nuclease active site and a helical domain. The residues subjected to site-directed mutagenesis are indicated by arrows.Back to article page