Figure 2

SDS/PAGE analysis of the purified recombinant wt TspGWI endonuclease. Lane M1, high protein marker (Sigma), bands marked: 205 kDa, myosin, 116 kDa, β-galactosidase, 97 kDa, phosporylase b; 66 kDa, bovine serum albumin; 55 kDa, glutamic dehydrogenase, 45 kDa, ovoalbumin; 36 kDa, glyceraldehyde-3-phosphate dehydrogenase; lane 1, control culture – crude lysate from E. coli expressing the cloned tspgwi gene, without induction (OD₆₀₀ = 0.7); lane 2, control culture – crude lysate from E. coli expressing the cloned tspgwi gene without induction, after 12 h of cultivation; lane 3, crude lysate from E. coli expressing the cloned tspgwi gene, before induction (OD₆₀₀ = 0.7); lane 4, crude lysate from E. coli expressing the cloned tspgwi gene, 3 h after induction; lane 5, crude lysate from E. coli expressing the cloned tspgwi gene, 6 h after induction; lane 6, crude lysate from E. coli expressing the cloned tspgwi gene, 12 h after induction; lane 7, purified, homogeneous recombinant wt TspGWI protein; lane M2, protein marker (Fermentas), bands marked: 200 kDa, 150 kDa, 120 kDa, 100 kDa, 85 kDa, 70 kDa, 60 kDa, 50 kDa, 40 kDa, 30 kDa, 25 kDa, 20 kDa.