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Figure 4 | BMC Molecular Biology

Figure 4

From: DNA methylation-histone modification relationships across the desmin locus in human primary cells

Figure 4

Histone H3 lysine (K) 27 tri-methylation (me3) is highly enriched at the DES CpG island in non-expressing PBMCs. nChIP experiments (Figure 2) were performed using antibodies against H3K27me3. (A) H3K27me3 of DES and the DES LCR in non-expressing PBMCs (grey bars). Non-specific binding was assessed by employing IgG (dark grey bars) and no antibody (stripped bars) samples as controls. Results are presented as fold-enrichment of IP samples over input DNA. Error bars represent the standard deviation about the mean fold-enrichment from at least 2 separate PCRs performed in duplicate from 2 independent nChIP experiments. (B) Illustration of DES and the DES LCR. Vertical arrows indicate the regions of high sequence conservation within each HS site of the LCR and the DES transcriptional start site (TSS) is represented by a horizontal arrow. Black rectangles denote the exons of DES. PCR amplicons (A-J) are shown by vertical black bars. Significant H3K27me3 enrichment was seen only at the DES promoter/CpG island.

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