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Figure 1 | BMC Molecular Biology

Figure 1

From: DNA methylation-histone modification relationships across the desmin locus in human primary cells

Figure 1

Microarray analysis for histone H3 and H4 acetylation across the DES LCR and ENCODE region ENr331 covering 500 kb of human chromosome 2q35. Chromatin (mono-tetranucleosomes) prepared using the nChIP approach was hybridized to a microarray tile, which covers a 500 kb region of chromosome 2q35 and encompasses the DES LCR and DES. Annotated genes taken from the UCSC genome browser http://genome.ucsc.edu are shown as black rectangles in each panel. (A) A peak of histone H3 acetylation can be observed at the beginning of the genes DES, SPEG and ACCN4. Note the grey bar indicating the position of APEG1. (B) Histone H4 acetylation appears over the entire gene rich region and is highly enriched across the DES promoter and LCR in muscle cells. The solitary peak of H4 acetylation downstream of the gene rich region (Panel F) was caused by an excess of Cy3 dye background staining on the corresponding part of the slide and therefore does not represent a true enrichment. Fold enrichment was obtained from an average ratio of two independent experiments.

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