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Table 2 Databases used for bioinformatic analysis and primers for PCR

From: Systematic cloning and analysis of autophagy-related genes from the silkworm Bombyx mori

Databases

SGD

http://www.yeastgenome.org/

SilkBase

http://papilio.ab.a.u-tokyo.ac.jp/silkbase

SilkDB

http://silkworm.genomics.org.cn/

GenBank

http://www.ncbi.nlm.nih.gov/Genbank/index.html

FlyBase

http://flybase.bio.indiana.edu/

SilkwormBLAST

http://silkworm.swu.edu.cn/silksoft/blast2-simple.html

Primers

BmATG8-FOR

CATGCCATGGATGAAATTCCAATACAA

BmATG8-REV

GCGTCGACTTAATTTCCATAGACAT

BmATG12-FOR,

CATGCCATGGATGAGTGATGAGAAA

BmATG12-REV

GTGAATTCTCAGCCCCAAGCTTG

Actin3-FOR

AGAGGTTCCGTTGTCCCG,

Actin3-REV

GGCGGTGATCTCCTTCTGCA

  1. aSGD, SilkBase, SilkDB, FlyBase and SilkwormBLAST were used for bioinformatic analysis, cDNA sequences were submitted to GenBank.
  2. bThe primers were used for semi-quantitative PCR analysis of BmATG8 and BmATG12.