Figure 4

Semi-quantitative PCR analyses of BmATG8 and BmATG12. A) Lane 1, Total RNA from newly hatched larva (N-H); Lane 2–8, Total RNA from silkgland of 1^st day to 7^th day fifth instar larvae (1–7); Lane 9–11, Total RNA from ASG(anterior silkgland), MSG(middle silkgland) and PSG(posterior silkgland) of the 8^th day fifth instar larvae. B) Quantification of the bands was performed by GIS 1D Software (Tanon, Shanghai, China) and the ratio of amplified target (At) to standard (As) was calculated for all samples.