Figure 7

A representative miRNA expression in microRNAarray analysis and predictive target genes. (a) Representative genes predicted to be commonly targeted by RGM249 miRNAs or siRNAs are shown. 4 miRNAs were predicted from the RGM249 sequence by miRBase http://microrna.sanger.ac.uk/sequences/index.shtml or miRNAMAP http://mirnamap.mbc.nctu.edu.tw/html/search.html and it was predicted that the miRNAs might inhibit the translation of genes involved in oxidation and RAS activation. As reported previously in association with hTERT expression, PRKCA, MAPK1, and AKT1 were included in the targeted genes. (b) Seven miRNAs upregulated more than twofold in RGM249 shRNA-transfectants by microRNAarray analysis performed between transfectants with LacZ shRNA and RGM249 shRNA are shown. Some of these 7 miRNAs have been indicated in the oncogenesis of oral squamous cell carcinoma (unpublished data). (c) Representative genes targeted by miRNAs which changed more than twofold when induced by shRGM249RNA are shown. The genes were predicted mainly using miRNAMAP. Three miRNAs were presumably targeted towards miRNA processing proteins, transcriptional factors, telomere-related genes, TERT-related genes, tumor suppressor-related genes, and cell cycle-related genes.