Figure 3

Outlines of cloned RGM249, a possible miRNA precursor or telomerase regulated gene. (a) RGM249 sequence is shown, and the sequences used for the siRNA and shRNA designs are underlined. (b) Predictive secondary structure of RGM249 was drawn by Vienna RNA Secondary prediction and comparison http://rna.tbi.univie.ac.at/cgi-bin/RNAfold.cgi. Bold line corresponds to the sequence used as the most functional siRNA or shRNA. The underlined parts show the sense and antisense sequences generating shRNA. Star (⋆) shows deletion of T or C in mt-1RGM249 or mt-2RGM249, respectively. (c) From the digestion by RNase III (Dicer), RGM249 mRNA generates 3 products ranging from 17 to 23 bp, suggesting that this gene may function as a noncoding precursor RNA gene which produces miRNAs.