Figure 2

A quantitative estimation in normal cells, cancer cells, and tissues. To quantify RGM249 mRNA expression in hepatocytes, hepatoma cells, and liver tissues, the copy number in 10 ng of total RNA was evaluated. (a) One-step real-time PCR was performed based on the linear correlation (P > 0.990) of the RNA controls. (b) RGM249 mRNA expression was examined in human representative organs using one-step real-time RT-PCR. The vertical line shows the copy number per 50 ng/μl total RNA (c) RGM249 mRNA expression in liver-related cells (from the left: adult liver tissues, fetal liver tissues, primary cultured hepatocytes [T], cytoplasm segment of primary hepatocytes [C], nuclear segment of primary hepatocytes [N], HLF cells, HMc-Li7 cells, HepG2 cells, and Alexander cells). (d) RGM249 mRNA expression in surgically resected liver tissues containing 33 pairs of hepatomas and adjacent noncancerous tissue. C: cancerous lesion, NC: adjacent noncancerous lesion. Cancerous lesions showed a significant upregulation of RGM249 mRNA, compared with noncancerous lesions, as determined by the t-test (P = 0.007) and paired t-test (P = 0.040). Box represents 95% confidence intervals.