Table 4 Primer pairs and amplicon sizes of the genes analyzed in this study.

ACCase

X77382

ACCase

Homomeric acetyl CoA carboxylase

F:5'AGGACTTGCCAATCTTCTAAAC3'

R:5'AGCTTCTTTCACCGTAGGACAC3'

AY538675

α-CT

alpha-carboxyltransferase

F:5'CTTGTCCACCCTATTCTGATTG3'

R:5'ATGTCCAGCTTAGATTTGAGGC3'

Z50868

β-CT

Beta-carboxyltransferase

F:5'CAGCAAGTTTGGGTATGTTGGG 3'

R:5'GTGAACCTTCAGGCACGGCTTT3'

AY034410

BC

Biotin carboxylase

F:5'AGGACCCATTCAAAGGATTCAG3'

R:5'GCTTGGAGGAACAACATAGTCG3'

Desaturase

AY642537

SAD

Stearoyl-ACP desaturase

F:5'GTTTACACTGCCAAAGACTATGCG3'

R:5'CCTGATTCTCGGAGTCAACCCAC3'

AY592975

FAD2

Oleate desaturase

F:5'AGGCGATAAAGCCGATACTTGG3'

R:5'CCTATCCGGTTCAACATAGATACACT3'

AY599884

FAD3

Linoleate desaturase

F:5'TTCCCACAAATCCCTCACTATCA3'

R:5'ACTTGCCACCAAACTTTCCACC3'

AY642535

FAD6

Oleate desaturase

F:5'ATCACATAAGCCCAAGGATACCG3'

R:5'TCGTCTTCATCAACCGCCAATT3'

Elongase

AJ007046

MCAT

Malonyl CoA-acyl carrier protein transacylase

F:5'ATCATAGGGTTGGACTCAGAAA 3'

R:5'ACTGCGTAGTTACCCGGACATA 3'

AF244519

KAS1

Beta-ketoacyl-ACP synthase 1

F:5'ACACGGTCGCAAACGAGAAGAA3'

R:5'GAAGATAATGGTGATGGAGCAG3'

AF244520

KAS2

Beta-ketoacyl-ACP synthase 2

F:5'GGAGTACCAAGCCCTTGCTCAC3'

R:5'TCCTTATGGCCTGCACAGTTGC3'

AF179854

KAS3

Beta-ketoacyl-ACP synthase 3

F:5'GGATGATGGGTTATTTAGTTTC3'

R:5'CCAAAGGGTAAAGCAGGAGAAG3'

AF009563

FAE1

Fatty acid elongase 1

F:5'GTCAGGCTTTAAGTGTAACAGTGCA3'

R:5'TTATTAGGACCGACCGTTTTGG3'

AF382146

KACD

3-keto-acyl-acp dehydratase

F:5'GATAGCGAAAATGGAAGGGAAAG3'

R:5'AAAGCAAAAGGCACGAGAACATA3'

AY196197

KCR2

3-ketoacyl-CoA reductase

F:5'TGAGTACAAGAAAAGTGGGATTG3'

R:5'GAGATGCCACTAAGAAAGATGCT3'

Thioesterase

BRU17098

FatA

Acyl-ACP thioesterase

F:5'GGGACCAATGGCTCTGCATCAT3'

R:5'GGCTTCTTTCTCCACAGGGTTG3'

DQ847275

FatB

Palmitoyl-ACP thioesterase

F:5'AGTTTGTGGGTGATGATGAATA3'

R:5'GCAAGGATAGGGTCAGAGTTCA3'

TAG synthesis

AF155224

DGAT2

Acyl-CoA: diacylglycerol acyltransferase

F:5'CATGACCTGATGAACCGCAAAG3'

R:5'ACGGCTACCAAAAGGATACAAAA3'

AF111161

LPAAT

Plastidial lysophosphatidic acid acyltransferase

F:5' CGAAGAGGCGAGAAACAAGATAG3'

R:5'TGGTTTAGCCTTCTCATTGTTCA3'

AY179560

AAPT1

Aminoalcoholphosphotransferase

F:5'TGGTGCTTCTTGGTTATTGTAT3'

F:5'GACCCTCGATGGTGAGTTTGA3'

Oil body protein

AY570250

Napin

1.7S oil body protein

R:5'CTTTGGATGCTCCTTTCAAGGT3'

F:5'GTAATCAATTTGGCCCTTAGCT3'

AY966447

Caleosin

Ca2+-binding oil body surface protein

R:5'CTCAAGATTCACAGGCATAAAC3'

F:5'CTGGGAGGCAAAGTTCAGGATA3'

X58000

Oleosin

oil body associated protein

R:5'CATGGCGTAATTTAGGTAGTGT3'

F:5'GAGGAGTCAGAGACCGCAGGA3'

M16860

Cruciferin

12S neutral oil bodyprotein

R:5'AAGGAAGCGAAGGATGGGGAGA3'

R:5'GGATTTGCATTATCCTCCCTTG3'

Housekeep genes

AF111812

β-actin

Housekeeping gene

F:5'CTGGAATTGCTGACCGTATGAG 3'

R:5'ATCTGTTGGAAAGTGCTGAGGG 3'

DQ097338

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

F:5'GCTATCAAGGAGGAATCTGAGGAC3'

R:5'CTTCACGAAATTGTCACTCAACG3'

Others

DQ167182

P 450

Cytochrome P450

F:5'ATGGATCTCGGGATCGGACAGT3'

R:5'GTCAAGCGATGACGGAGCAAAA3'

X93015

GKTP

Glyoxysomal beta-ketoacyl-thiolase precursor

F:5'GTTGGTCCAGCAGTTGCCATTC3'

R:5'CGCCTCCGTTGACATTGATTTT3'

AJ223497

PEPC

Phosphoenolpyruvate carboxylase

F:5'GGTTGGGTTTATTGGTTTGTTTATG3'

R:5'ATTCCCTTGCTCGGTTTTGTTA3'

AJ271162

AGPase

ADP-glucose pyrophosphorylase small subunit

F:5'AGACACCACCACCCCGTTTGAC3'

R:5' TTTAGGGATAAGGCAGGAGGAT3'

AB041622

BcRK6

Receptor kinase 6

F:5'AGGTTAAGTGACGGGCAAGAAA3'

R:5'TTGAACGCAACAGCCAAGAAGT3'

AY065839

SUC1

Sucrose transporter

F:5'GCCAAGGACTGTCGTTAGGAGTTT3'

R:5'TGCGATTGCTCCGACTATAAATG3'