Skip to main content
Figure 7 | BMC Molecular Biology

Figure 7

From: Key role of the 3' untranslated region in the cell cycle regulated expression of the Leishmania infantum histone H2A genes: minor synergistic effect of the 5' untranslated region

Figure 7

Analysis of the CAT cell cycle regulated expression in different constructs. (A) Ratio between the de novo CAT synthesis (normalised to the total amount of CAT) and number of cells in the S phase (3 h after HU removal) determined by three independent assays in cell lines transfected with pXCATHSP83, pXCAT5'III/3'I, pXCAT5'H2A and pXCAT3'H2A constructs. (B) Graphic showing the percentages of CAT mRNA on polysomes in the same cell lines. Data were obtained from the densitometric analysis of the blots performed with the RNAs separated on sucrose gradients from cell lines transfected with pXCATHSP83, pXCAT5'III/3'I, pXCAT5'H2A and pXCAT3'H2A constructs at G1 and S phase for three independent assays.

Back to article page