Skip to main content
Figure 1 | BMC Molecular Biology

Figure 1

From: Key role of the 3' untranslated region in the cell cycle regulated expression of the Leishmania infantum histone H2A genes: minor synergistic effect of the 5' untranslated region

Figure 1

DNA constructs. (A) Schematic representation of the two Leishmania infantum H2A loci. The names of the genes are indicated below the maps. The locations of the different 5'(I-IV) and 3' UTR (I-III) are indicated on the diagram. Due to the sequence similarity 5'UTR-I and -II are represented as white boxes and 5'UTR-III and -IV are represented as black boxes. These sequences are in the EMBL databases under the accession numbers AJ419625 and AJ419627. (B) Generic representation of the pX63NEO recombinant vector. Position of the Bam HI cut sites employed in the cloning are indicated as B. Positions of the 5'UTR + upstream regions (UPR) and 3' UTR + downstream regions (DNR) relative to CAT gene coding region are indicated. Transcriptional directions of the NEO and CAT genes are as indicated. (C) Schematic outlines of the constructs used in this work indicating the name of the clone and the type of 5'UTR+UPR and 3'UTR+DNR included around the CAT coding region. (D) Northern blots of RNA from promastigotes stably transfected with the different constructs and hybridized with radiollabeled oligonucleotide complementary to the 3' terminal region of CAT gene coding region. Ethidium bromide staining of the corresponding gel is also shown (rRNA panel).

Back to article page