Table 2 Binding affinity of SLM-2 for selected and mutated RNA ligands
From: The STAR RNA binding proteins GLD-1, QKI, SAM68 and SLM-2 bind bipartite RNA motifs
Ligand | Sequence | Binding affinity |
|---|---|---|
SRE-4wt | GGUUCUAUAAA AAUUUUCACUAUCCUAUUAA CAGUUCC | 16.3 +++ |
SRE-4m1 | GGUUCUAccccAAUUUUCACUAUCCUAccccCAGUUCC | >1000 - |
SRE-4m2 | GGUUCUAccccAAUUUUCACUAUCCUAUUAA CAGUUCC | >1000 - |
SRE-4m3 | GGUUCUAUAAA AAUUUUCACUAUCCUAccccCAGUUCC | >1000 - |
SRE-4m4 | GGUUCUAUA cccccUUUCACUAUCCUAUUAA CAGUUCC | >1000 - |
SRE-7wt | AUCGUGGUGGGCGGUUAA UUUGGAUUUCUUGAGCUUAUGGCUUUUAA UAUGGG | 30.3 +++ |
SRE-7m1 | AUCUUguucucCGCUUAA UUUGGAUUUCUUGAGCUUAUGGCUUUUAA UAUGGG | 45.2 +++ |
SRE-7m2 | AUCGUaauaaaCaaUUAA UUUGGAUUUCUUGAGCUUAUGGCUUUUAA UAUGGG | 14.5 +++ |
SRE-7m3 | AUCGUGGUGGGCGGUUAA UUUGGAUUUCUUGAGCUUAUGGCUcgacgCAUGGG | >1000 - |
SRE-7d1 | AUCGUGGUGGGCGGUUAA UUUGGAUUU | >1000 - |
SRE-7d2 | GUGGGCGGUUAA UUUGGAUUUCUUGAG | >1000 - |
SRE-7d3 | GGUUAA UUUGGAUUUCUUGAGCUUAUG | >1000 - |
SRE-7d4 | UUUGGAUUUCUUGAGCUUAUGGCUUUU | >1000 - |
SRE-7d5 | UUUCUUGAGCUUAUGGCUUUUAA UAUGG | >1000 - |
SRE-7d6 | GGAUUUCUUGAGCUUAUGGCUUUUAA U | >1000 - |
SRE-7d7 | UAA UUUGGAUUUCUUGAGCUUAUGGCU | >1000 - |
SRE-7d8 | GGUUAA UUUGGAUUUCUUGAGCUUAUGGCUUUU | >1000 - |
SRE-7d9 | GGUUAA UUUGGAUUUCUUGAGCUUAUGGCUUUUAA UAUGG | 22.3 +++ |
SRE-7d9m1 | GGUU ccccUGGAUUUCUUGAGCUUAUGGCUUU ccccAUGG | >1000 - |
SRE-7d9m2 | GGUU ccUUUGGAUUUCUUGAGCUUAUGGCUUUUAA UAUGG | ≅400 + |
SRE-7d9m3 | GGUUAA UCUGGAUUUCUUGAGCUUAUGGCUUUU ccUAUGG | ≅ 400 + |