Figure 1From: Validation of commonly used reference genes for sleep-related gene expression studiesRepresentative image of electrophoresis of total RNA in agarose gel. Total RNA extracted from brain of controls (1, 2, 3) and rats subjected to paradoxical sleep deprivation without (4, 5, 6) or with sleep recovery (7, 8, 9) was fractioned in agarose gel 1%. Approximately 1.5 μg of RNA was loaded for each sample. Intact 28 S and 18 S rRNA were observed without higher molecular weight molecules.Back to article page