Figure 4

LsTryp10 mRNA localization and histology. A. Adult female L. salmonis with eggstrings, broken white line indicates location of section shown in B. e: eggstrings. B. Section of adult female L. salmonis. ov: ovary; ovd: oviduct. C. Immature oocytes (o) in the oviduct. D. Transition from immature oocytes in the oviduct (C) to mature oocytes in the genital segment (E). E. Mature oocytes (mo) in the genital segment after being filled with droplets and granules. Note that the chromosomes appear to be in the pachytene or diplotene stage both in the immature and mature oocytes (arrows in C and E). ISH using antisense (F, H, J) and sense (G, I, K) RNA probes show that LsTryp10 mRNA is present in the ovary (ov), in immature oocytes (io) in the oviduct (ovd) as well as in mature oocytes in the unfertilized eggstring (ufe). In the mature oocytes LsTryp10 mRNA is localized along the oocyte periphery (arrowheads in F, corresponding locations in negative control (G) also marked with arrowheads). Bar = 1 cm in A, 100 μm in C-K.