Figure 4
From: TCF/β-catenin plays an important role in HCCR-1 oncogene expression
TheTcf1 site on the HCCR-1 promoter was sensitive to the GSK-3β inhibitor, LiCl. The truncated constructs (A) or mutated constructs (B) of the HCCR-1 promoter region were transfected into K562 cells with pRL-CMV as an internal control. Cells were then treated with or without 5 mM LiCl for 24 hours and subjected to dual luciferase analysis (n > 4 per construct).