Figure 3

DNA-binding activity at the Tcf1 site. EMSA were performed using K562 nuclear extracts and dsDNA harboring either a consensus site for HCCR-1 wild-type Tcf1, Tcf2 element (A), and a mutated sequence mTcf1 (B). To show the specificity of the bands, 10 times molar excess of cold double-stranded oligonucleotide carrying the consensus Tcf1 and Tcf2 sites was used for competition or Ery for non-competition. B1 indicates the DNA-protein complex with probe Tcf1 and B2 indicates less specific DNA-protein complex. The presence or absence of a component in the assay is indicated by a "+" or "-", respectively.