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Figure 5 | BMC Molecular Biology

Figure 5

From: Thrombin induces Egr-1 expression in fibroblasts involving elevation of the intracellular Ca2+ concentration, phosphorylation of ERK and activation of ternary complex factor

Figure 5

Role of EGF receptor transactivation in thrombin signaling. 39M1-81 cells (A) or 1321N1 glioma cells (B) were preincubated with AG1478 (0.5 μM) or vehicle and then stimulated with either thrombin (1 U/ml) or carbachol (100 μM) for 1 hour. Nuclear extracts were prepared and subjected to Western blot analysis using an antibody directed against Egr-1. As a control, cells were stimulated with EGF (10 ng/ml) in the presence or absence of AG1478 (A, lower panel). (C) 39M1-81 cells were preincubated with AG1478 (0.5 μM) or vehicle and then stimulated with either thrombin (1 U/ml) or carbachol (100 μM) for 5 min. Whole cell extracts were prepared and subjected to Western blot analysis. The blots were incubated with a monoclonal antibody directed against the phosphorylated active forms of ERK1 and ERK2.

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