Figure 6

Putative Orf1-binding mRNA motif. (A) Predicted mRNA secondary structures reveled by FOLDALIGN [67, 68] in abiD1 mRNA (nucleotide positions are 7341–7362; 7518–7539; GenBank Accession Number AF116286) and (B) phage bIl66 mid-infection induced M-operon mRNA (nucleotide positions are 566–591; GenBank Accession Number L35175) and L. lactis IL1403 cold shock inducible genes osmC, hslA, clpX, llrC and pgmB mRNAs (nucleotide positions are 68675–68698; 502344–502363; 1163846–1163866; 403740–403763 and 442024–442049, respectively; GenBank Accession Number NC_002662). Gene names are indicated. (C) Binding of Orf1 to M-operon and osmC RNAs. Experiments were performed with increasing amounts of Orf1 (0; 0.05 μM; 0.11 μM; 0.22 μM) in the same conditions as above.