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Figure 2 | BMC Molecular Biology

Figure 2

From: Activation of mRNA translation by phage protein and low temperature: the case of Lactococcus lactis abortive infection system AbiD1

Figure 2

Translation of the abiD1 mRNA. (A) MFOLD [44] predicted secondary structure of the abiD1 translation initiation region (TIR). The sequence is presented from the transcriptional start (+ 1) to the abiD1 AUG start codon. Ribosome binding site (RBS) and the poly-U stretch are boxed. Arrows show the 5' ends of different TIRs used. The start of luxAB gene in fusion constructs is indicated. (B) Translation of abiD1 TIR:luxAB fusions. The background level of luciferase activity was measured in the presence of plasmid pJIM1715 [50]. Experiments were performed at 30°C. Results are means of 6–10 independent experiments. Luciferase activity is shown in arbitrary light units (lux/OD unit at an OD600 of 0.4).

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