Figure 1

Transcriptional analysis of the abiD1 gene. (A) Schematic organization of the abiD1 gene region. Bent arrows and circles denote promoter and terminator sequences, respectively. Position of oligonucleotides used for Northern hybridization (1) and quantitative RT-PCRs are indicated by tail-less arrows. Transcripts initiated at the abiD1 promoter are shown by solid (50 b) and broken (3.6 kb) lines. (B) Northern hybridization results. RNA was extracted from IL1403 AbiD1+ cells grown with or without Cm. Hybridization was performed with oligonucleotide n°1. (C) Quantitative RT-PCRs. abiD1 transcript was followed in IL1403 AbiD1+ cells. Samples were taken before (time 0), 10 and 20 min after infection with bIL66 phage. Amount of abiD1 transcript was normalized to L. lactis tuf transcript level. Values shown are means of 9–15 measurements, expressed in arbitrary units.