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Figure 1 | BMC Molecular Biology

Figure 1

From: Revised Mimivirus major capsid protein sequence reveals intron-containing gene structure and extra domain

Figure 1

Schematic organisation and expression of the full length L425 gene. (A) L425 gene (nt 560926 – 557533 position on the APM genomic DNA) showed three coding sequences, boxed in grey, separated by two intron sequences. Organisation of the corresponding cDNA and 593AA full length protein are shown below. Locations of the former D13L capsid coding sequence and corresponding protein are indicated by dotted lines. (B) PCR on APM genomic DNA (lane 1) and RT-PCR analysis on RNA extracted from uninfected (lane 2) or APM-infected amoebae at 0, 2, 4, 8 and 16 h p.i. (lanes 3–7, respectively) using 5' Q5UQL7NcoIF and 3' Q5UQL7SmaIR primers. Left lane: DNA size marker.

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BMC Molecular Biology

ISSN: 1471-2199

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